human pancreatic cancer cell lines cfpac 1  (ATCC)

Journal: Metabolites

Article Title: Ubiquitous Aberration in Cholesterol Metabolism across Pancreatic Ductal Adenocarcinoma

doi: 10.3390/metabo12010047

Figure Lengend Snippet: Changes in cholesterol synthesis pathways in pancreatic cancer. ( A ) A schematic of pancreatic cancer progression. ( B ) List of some of the enzymes and metabolic intermediates involved in the cholesterol biosynthesis pathway. ( C , D ) Heatmaps of alterations in cholesterol synthesis enzymes in pancreatic cancer. Color gradient: red, black, to green in each row of the heatmaps indicates relative differences in the gene expression patterns as indicated by the scale provided below the heatmap. The significant changes are marked with * <0.05; ** <0.01; *** <0.001.

Article Snippet: Human pancreatic cancer cell lines (CFPAC1, SW1990) and normal pancreatic cell line HPNE were obtained from American Type Culture Collection, whereas T3M4 and COLO357 were obtained from our collaborator.

Techniques: Gene Expression

Journal: Metabolites

Article Title: Ubiquitous Aberration in Cholesterol Metabolism across Pancreatic Ductal Adenocarcinoma

doi: 10.3390/metabo12010047

Figure Lengend Snippet: Sterol changes in human pancreatic tissues. ( A ) Mean and standard error plots showing sterol levels in normal human pancreatic tissues and pancreatic cancer tumors (PC) normalized with the protein content of respective tissue specimen. ( B ) Mean and standard error plots showing sterol levels in normal human pancreatic tissues, pancreatic tumors (PC), and metastatic pancreatic cancer (Met PC) tissues normalized to protein content of the respective tissue specimen. * = 0.0622, indicates significant difference in cholesterol comparing normal vs. PC obtained through Welch’s t -test.

Article Snippet: Human pancreatic cancer cell lines (CFPAC1, SW1990) and normal pancreatic cell line HPNE were obtained from American Type Culture Collection, whereas T3M4 and COLO357 were obtained from our collaborator.

Techniques:

Journal: Metabolites

Article Title: Ubiquitous Aberration in Cholesterol Metabolism across Pancreatic Ductal Adenocarcinoma

doi: 10.3390/metabo12010047

Figure Lengend Snippet: Serum cholesterol levels are high in human pancreatic cancer. ( A ) Mean and standard deviation plots showing sterol levels in human sera collected from healthy (normal) and patients with pancreatic ductal adenocarcinoma (PDAC) normalized with the volume of the specimen. ( B ) Mean and SE plots showing sterol levels in human normalized to serum volume and classified based on healthy (normal) and stagewise progression of pancreatic cancer, including stages 2a, 2b, 3, and 4, respectively. * indicates p < 0.05 obtained through unpaired t- test.

Article Snippet: Human pancreatic cancer cell lines (CFPAC1, SW1990) and normal pancreatic cell line HPNE were obtained from American Type Culture Collection, whereas T3M4 and COLO357 were obtained from our collaborator.

Techniques: Standard Deviation

Journal: Metabolites

Article Title: Ubiquitous Aberration in Cholesterol Metabolism across Pancreatic Ductal Adenocarcinoma

doi: 10.3390/metabo12010047

Figure Lengend Snippet: Sterol levels increase in GEMM models of pancreatic cancer: Mean and standard deviation plots showing sterol levels in normal, Kras G12D ; PdxCre (KC) and Kras G12D ; Trp53 R172H ; PdxCre (KPC) pancreatic tissues. *, ** and *** indicate p < 0.05, 0.01 and 0.001 respectively, obtained through unpaired t- test.

Article Snippet: Human pancreatic cancer cell lines (CFPAC1, SW1990) and normal pancreatic cell line HPNE were obtained from American Type Culture Collection, whereas T3M4 and COLO357 were obtained from our collaborator.

Techniques: Standard Deviation

Journal: Metabolites

Article Title: Ubiquitous Aberration in Cholesterol Metabolism across Pancreatic Ductal Adenocarcinoma

doi: 10.3390/metabo12010047

Figure Lengend Snippet: Sterol levels in pancreatic cancer cell lines alter with lipid deprivation. Mean and standard deviation bar plots depicting sterol quantification from immortalized pancreatic epithelial cell line (HPNE) and pancreatic ductal carcinoma cell lines (CFAPC1, SW1990, T3M4, and COLO357) grown in a delipidated medium are shown in panel ( A ), and N2-supplemented medium is shown in panel ( B ). *, **, and *** indicate p < 0.01, <0.05, and <0.001, respectively, which were obtained through one-way ANOVA analysis.

Article Snippet: Human pancreatic cancer cell lines (CFPAC1, SW1990) and normal pancreatic cell line HPNE were obtained from American Type Culture Collection, whereas T3M4 and COLO357 were obtained from our collaborator.

Techniques: Standard Deviation